全文获取类型
收费全文 | 880篇 |
免费 | 109篇 |
国内免费 | 30篇 |
专业分类
化学 | 1004篇 |
综合类 | 6篇 |
物理学 | 9篇 |
出版年
2023年 | 6篇 |
2022年 | 23篇 |
2021年 | 41篇 |
2020年 | 52篇 |
2019年 | 38篇 |
2018年 | 26篇 |
2017年 | 27篇 |
2016年 | 43篇 |
2015年 | 41篇 |
2014年 | 40篇 |
2013年 | 52篇 |
2012年 | 57篇 |
2011年 | 34篇 |
2010年 | 50篇 |
2009年 | 50篇 |
2008年 | 46篇 |
2007年 | 50篇 |
2006年 | 45篇 |
2005年 | 37篇 |
2004年 | 37篇 |
2003年 | 45篇 |
2002年 | 97篇 |
2001年 | 13篇 |
2000年 | 3篇 |
1999年 | 13篇 |
1998年 | 6篇 |
1997年 | 10篇 |
1996年 | 4篇 |
1995年 | 9篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1980年 | 1篇 |
1979年 | 2篇 |
排序方式: 共有1019条查询结果,搜索用时 109 毫秒
131.
132.
133.
von Nussbaum F Brands M Hinzen B Weigand S Häbich D 《Angewandte Chemie (International ed. in English)》2006,45(31):5072-5129
To create a drug, nature's blueprints often have to be improved through semisynthesis or total synthesis (chemical postevolution). Selected contributions from industrial and academic groups highlight the arduous but rewarding path from natural products to drugs. Principle modification types for natural products are discussed herein, such as decoration, substitution, and degradation. The biological, chemical, and socioeconomic environments of antibacterial research are dealt with in context. Natural products, many from soil organisms, have provided the majority of lead structures for marketed anti-infectives. Surprisingly, numerous "old" classes of antibacterial natural products have never been intensively explored by medicinal chemists. Nevertheless, research on antibacterial natural products is flagging. Apparently, the "old fashioned" natural products no longer fit into modern drug discovery. The handling of natural products is cumbersome, requiring nonstandardized workflows and extended timelines. Revisiting natural products with modern chemistry and target-finding tools from biology (reversed genomics) is one option for their revival. 相似文献
134.
135.
136.
This review covers the main aspects concerning the chemistry, the biological activity and the analytical determination of oxazolidinones, the only new class of synthetic antibiotics advanced in clinical use over the past 50 years. They are characterized by a chemical structure including the oxazolidone ring with the S configuration of substituent at C5, the acylaminomethyl group linked to C5 and the N-aryl substituent. The synthesis of oxazolidinones has gained increasing interest due to their unique mechanism of action that assures high antibiotic efficiency and low susceptibility to resistance mechanisms. Here, the main features of oxazolidinone antibiotics licensed or under development, such as Linezolid, Sutezolid, Eperezolid, Radezolid, Contezolid, Posizolid, Tedizolid, Delpazolid and TBI-223, are discussed. As they are protein synthesis inhibitors active against a wide spectrum of multidrug-resistant Gram-positive bacteria, their biological activity is carefully analyzed, together with the drug delivery systems recently developed to overcome the poor oxazolidinone water solubility. Finally, the most employed analytical techniques for oxazolidinone determination in different matrices, such as biological fluids, tissues, drugs and natural waters, are reviewed. Most are based on HPLC (High Performance Liquid Chromatography) coupled with UV-Vis or mass spectrometer detectors, but, to a lesser extent are also based on spectrofluorimetry or voltammetry. 相似文献
137.
Meghdad Pirsaheb Hiva Hosseini Hasan Mohamadi Sorkali Negar Noori 《International journal of environmental analytical chemistry》2019,99(2):112-123
In the present study, a new method for extraction and preconcentration of amoxicillin and ceftriaxone was used in hospitalised sewage samples, called vortex-assisted liquid-phase microextraction based on the solidification of deep eutectic solvent. Samples were analysed by high-performance liquid chromatography–ultraviolet detection after preparation and extraction. In this method, the new deep eutectic solvent is used as the extraction solvent, which is obtained from the combination of 1-decyl-3-methylimidazolium chloride and n-butanoic acid. The important advantages of this novel extraction solvent include material stability, low density and good freezing point near room temperature. Under the optimum conditions, enrichment factors are in the range of 164–172. Repeatability and reproducibility of the method based on seven replicate measurements of 50.0 µg L?1 of the target analytes in analysed samples were in the range of 2.1–3.5% and 3.8–5.2%, respectively. The limit of detections and linearity are in the range of 0.005–0.10 and 3–600 µg L?1, respectively. The method was successfully applied for the determination of amoxicillin and ceftriaxone in the real sewage samples. The relative recoveries of sewage samples spiked with amoxicillin and ceftriaxone are 91–107%. 相似文献
138.
QuEChERS/超高效液相色谱-串联质谱法快速测定土壤中19种氟喹诺酮类抗生素残留 总被引:2,自引:0,他引:2
建立了一种基于QuEChERS前处理技术,结合超高效液相色谱-串联质谱(UPLC-MS/MS)和内标法,快速测定土壤中19种氟喹诺酮类抗生素(FQs)残留的分析方法。5. 0 g土壤样品添加200μg/kg基质匹配同位素内标后,经20 m L 0. 1 mol/L EDTA-McIlvaine缓冲液和乙腈混合溶剂(体积比1∶1)提取,基质分散固相萃取(150 mg无水MgSO4、15 mg PSA、15 mg C18)净化后,采用UPLC-MS/MS进行测定。质谱分析采用电喷雾电离,正离子扫描,多反应监测模式。在优化条件下,19种抗生素的相关系数(r~2)为0. 992~0. 998,检出限为0. 2~1. 0μg/kg,定量下限为1. 0~5. 0μg/kg;在10、50、200μg/kg加标水平下的回收率为65. 2%~104%,相对标准偏差(n=5)不大于14%。该方法操作简单快速、准确度高,适用于土壤中氟喹诺酮类抗生素残留的检测。 相似文献
139.
A robust 4,8-connected Tb-based metal-organic framework (Tb-MOF) with paddle wheel-shaped {Tb2(COO)4} subunits extended by C3-symmetric 3,3',3''-[1,3,5-benzenetriyltris(carbonylimino)]tris-benzoate connector was hydrothermally synthesized, showing highly environmental stability, good dispersion and intense green emission in water system. Resulting critically from the well suppressed absorption towards the excitation energy, the Tb-MOF exhibits rapid and efficient fluorescent response towards nitroimidazole antibiotics with strong quenching constants and low detection limits of 1.59 × 104 m –1 and 2.4 μM for metronidazole as well as 1.62 × 104 m –1 and 2.9 μM for dimetridazole. Moreover, the sensitive and selective identification of the Tb-MOF has strong anti-interference and excellent regeneration ability, which endows the promising applications of the Tb-MOF as fluorescent sensing materials. 相似文献
140.
Tobramycin and Nebramine as Pseudo‐oligosaccharide Scaffolds for the Development of Antimicrobial Cationic Amphiphiles 下载免费PDF全文
Yifat Berkov‐Zrihen Ido M. Herzog Raphael I. Benhamou Dr. Mark Feldman Kfir B. Steinbuch Pazit Shaul Dr. Shachar Lerer Dr. Avigdor Eldar Dr. Micha Fridman 《Chemistry (Weinheim an der Bergstrasse, Germany)》2015,21(11):4340-4349
Antimicrobial cationic amphiphiles derived from aminoglycoside pseudo‐oligosaccharide antibiotics interfere with the structure and function of bacterial membranes and offer a promising direction for the development of novel antibiotics. Herein, we report the design and synthesis of cationic amphiphiles derived from the pseudo‐trisaccharide aminoglycoside tobramycin and its pseudo‐disaccharide segment nebramine. Antimicrobial activity, membrane selectivity, mode of action, and structure–activity relationships were studied. Several cationic amphiphiles showed marked antimicrobial activity, and one amphiphilic nebramine derivative proved effective against all of the tested strains of bacteria; furthermore, against several of the tested strains, this compound was well over an order of magnitude more potent than the parent antibiotic tobramycin, the membrane‐targeting antimicrobial peptide mixture gramicidin D, and the cationic lipopeptide polymyxin B, which are in clinical use. 相似文献